Biological Synthesis of 7-O-Methyl Apigenin from Naringenin Using Escherichia coli Expressing Two Genes | DBpia (2025)

Within the secondary metabolite class of flavonoids, whichconsist of more than 10,000 known structures, flavonesdefine one of the largest subgroups. The diverse functionof flavones in plants as well as their various roles in theinteraction with other organisms offers many potentialapplications including in human nutrition and pharmacology.We used two genes, flavone synthase (PFNS-1) that convertsnaringenin into apigenin and flavone 7-O-methyltransferasePOMT-7) that converts apigenin into 7-O-methyl apigenin,to synthesize 7-O-methyl apigenenin from naringenin.The PFNS-1 gene was subcloned into the E. coli expressionvector pGEX and POMT-7 was subcloned into the pRSFvector. Since both constructs contain different replicationorigins and selection markers, they were cotransformedinto E. coli. Using E. coli transformants harboring bothPFNS-1 and POMT-7, naringenin could be converted into7-O-methyl apigenin, genkwanin.

Within the secondary metabolite class of flavonoids, whichconsist of more than 10,000 known structures, flavonesdefine one of the largest subgroups. The diverse functionof flavones in plants as well as their various roles in theinteraction with other organisms offers many potentialapplications including in human nutrition and pharmacology.We used two genes, flavone synthase (PFNS-1) that convertsnaringenin into apigenin and flavone 7-O-methyltransferasePOMT-7) that converts apigenin into 7-O-methyl apigenin,to synthesize 7-O-methyl apigenenin from naringenin.The PFNS-1 gene was subcloned into the E. coli expressionvector pGEX and POMT-7 was subcloned into the pRSFvector. Since both constructs contain different replicationorigins and selection markers, they were cotransformedinto E. coli. Using E. coli transformants harboring bothPFNS-1 and POMT-7, naringenin could be converted into7-O-methyl apigenin, genkwanin.

#Flavone synthase #genkwanin #O-methyltransferase

Balunas, M. J. / 2005 / Drug discovery from medicinal plants / Life Sci 78 : 431 ~ 441 Bong Gyu Kim / 2008 / Accumulation of Flavonols in Response to Ultraviolet-B Irradiation in Soybean Is Related to Induction of Flavanone 3-beta-Hydroxylase and Flavonol Synth Croteau, R. / 2000 / Natural products (secondary metabolites). in: Biochemistry and Molecular Biology of Plant / American Society of Plant Physiologists : 1250 ~ 1318 Harborne, J. B. / 2000 / Advances in flavonoid research since 1992 / Phytochemistry 55 : 481 ~ 504 Julsing, M. K. / 2006 / Combinatorial biosynthesis of medicinal plant secondary metabolites / Biomolec. Eng 23 : 265 ~ 279

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